Molecular Identification and Detection of Phytophthora ramorum
نویسندگان
چکیده
The genus Phytophthora comprises over 70 described species, however many new species have been reported recently as a result of the discovery of previously undetected species or by the hybridization of known species. Phytophthora ramorum, one of the new Phytophthora species, is considered a high phytosanitary risk because it is currently responsible for largescale oak mortality in coastal California forests. In Europe, the disease occurs mainly in nurseries on Rhododendron, Viburnum and Camellia. However, in landscapes, infected trees of Quercus rubra, Q falcata, Q. ilex, Aesculus hippocastanum and Fagus sylvatica were reported recently on sites previously identified with infected Rhododendron plants. In addition, numerous nurseries in the U. S. have recently been diagnosed with infected Rhododendron and Camellia spp.
منابع مشابه
Diagnostic Values and Utility of Immunological, Morphological, and Molecular Methods for In Planta Detection of Phytophthora ramorum.
ABSTRACT In this study, six methods for the detection of Phytophthora ramorum in planta were compared using naturally infested rhododendron plant material. The methods included two immunological methods, one an enzyme-linked immunosorbent assay (ELISA) and the other using a lateral flow format (LFD). Three molecular tests based on the polymerase chain reaction (PCR) using TaqMan chemistry also ...
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Here we present genome sequences for twelve isolates of the invasive pathogen Phytophthora ramorum EU1. The assembled genome sequences and raw sequence data are available via BioProject accession number PRJNA177509. These data will be useful in developing molecular tools for specific detection and identification of this pathogen.
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Molecular techniques have been developed for detection and identification of P. ramorum and other Phytophthora species that are based on the mitochondrially encoded sequences. One technique uses a Phytophthora genus specific primer to determine if a Phytophthora species is present, followed by a nested amplification with species-specific primers to determine if the pathogen is P. ramorum or one...
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Given the importance of Phytophthora ramorum from a regulatory standpoint, it is imperative that molecular markers for pathogen detection are fully tested to evaluate their specificity in detection of the pathogen. In an effort to evaluate 11 reported diagnostic techniques, we assembled a standardized DNA library using accessions from the World Phytophthora Genetic Resource Collection for 315 i...
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ABSTRACT Sudden oak death, caused by Phytophthora ramorum, is a severe disease that affects many species of trees and shrubs. This pathogen is spreading rapidly and quarantine measures are currently in place to prevent dissemination to areas that were previously free of the pathogen. Molecular assays that rapidly detect and identify P. ramorum frequently fail to reliably distinguish between P. ...
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ABSTRACT The timely and accurate detection of pathogens is a critical aid in the study of the epidemiology and biology of plant diseases. In the case of regulated organisms, the availability of a sensitive and reliable assay is essential when trying to achieve early detection of the pathogen. We developed and tested a real-time, nested polymerase chain reaction (PCR) assay for the detection of ...
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